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Exposure to oxidized low-density lipoprotein reduces activable ras protein in vascular endothelial cells

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Summary

Oxidized low-density lipoprotein (ox-LDL) has been shown to alter the migratory and proliferative activities of the vascular endothelial cells (EC) in response to serum and growth factors. The mechanism underlying the antiproliferative effect of ox-LDL on vascular EC has not been fully elucidated. In this report, we show that exposure of vascular EC to ox-LDL results in a marked reduction of the membrane-associated Ras protein. Further study shows that in ox-LDL-treated EC, reduction of the membrane-associated Ras protein is correlated with a reduced amount of active Ras (Ras-guanosine triphosphate), indicating that the Ras signaling pathway is attenuated. The attenuation of the Ras signaling pathway in ox-LDL-treated EC may thus be responsible for the retarded response to the mitogenic stimulation of serum and growth factors.

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Authors and Affiliations

  1. Center of General Study, Chang Gung University, Taoyuan, Taiwan, ROC

    Shu-Er Chow

  2. Department of Physiology, College of Medicine, Chang Gung University, Taoyuan, Taiwan, ROC

    Wing-Keung Chu & Jan-Kan Chen

  3. Department of Biology, National Taiwan Normal University, Taipei, Taiwan, ROC

    Stephen H. Shih

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  1. Shu-Er Chow
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Correspondence to Jan-Kan Chen.

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Chow, SE., Chu, WK., Shih, S.H. et al. Exposure to oxidized low-density lipoprotein reduces activable ras protein in vascular endothelial cells. In Vitro Cell.Dev.Biol.-Animal 38, 320–325 (2002). https://doi.org/10.1290/1071-2690(2002)038<0320:ETOLDL>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2002)038<0320:ETOLDL>2.0.CO;2

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